Regulation of σB-Dependent Biofilm Formation in Staphylococcus aureus through Strain-Specific Signaling Induced by Diosgenin

Staphylococcus aureus is a commensal skin bacterium and causative agent of infectious diseases. Biofilm formation in S. mechanism that facilitates the emergence resistant strains. This study proposes for regulation biofilm through strain-specific physiological changes induced by plant steroid diosgenin. A comparison diosgenin-induced expression regulatory genes associated with revealed intracellular mechanisms involved formation. Diosgenin reduced ATCC 6538 methicillin-resistant (MRSA) CCARM 3090 39% 61%, respectively. Conversely, it increased 29213 MRSA 3820 186% 582%, Cell surface hydrophobicity extracellular protein carbohydrate contents changed manner response to An assessment gene diosgenin treatment decreased icaA spa RNAIII, agrA, sarA, sigB 3090; however, contrasting were noted 3820. These results suggest activated sequentially increases RNAIII. RNAIII decreases spa, surface-associated adhesion factor. additional an unknown regulator icaA. turn icaA, which synthesis polysaccharide intercellular adhesins ultimately inhibits By assessing signals without mutation, this elucidated signal transduction regulate based on changes.